Replication of HSV-1 in DCs isolated from BALB/cmice. Panel A. Subconfluent monolayers of DCs and RS cells were infected with 10 or 1 PFU per cell of McKrae and the virus yield determined at the indicated times by standard plaque assays as described in Materials and Methods. Panel B. Cells were infected at 10 PFU per cell and virus allowed to attach for 1 h at 4°C or 37°C. Monolayers were washed 3× and total virus remaining associated with the cells was determined by plaque assay as described in Materials and Methods. In both panels each point represents the mean ± SEM (n = 16) from two to 4 separate experiments.