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Table 1 Cytopathic effects (CPE) produced by parental and progeny virus strains in mammalian cell lines.

From: In vitro host range, multiplication and virion forms of recombinant viruses obtained from co-infection in vitro with a vaccinia-vectored influenza vaccine and a naturally occurring cowpox virus isolate

Cytopathic Effects (CPE)a

Cell line

Species/tissue

CPXV-NOH1

MVA-HANP

Rec1

Rec 2

Rec 3

Rec 3a

Rec 3b

IEC-6

Rat/small intestine; normal

+++

++

+++

++++

++

++++

++++

BHK-21

Hamster syrian/kidney; normal

++

+++

++++

++++

++

++++

++++

Caco-2

Human/colon; colorectal adenocarcinoma

+++

-

+

++

+++

+++

++++

H411E

Rat/liver; hepatoma

++

+

++

+++

+

+++

+++

FHs74int

Human/small intestine; normal

+++

-

++

+

++

+++

++++

Hutu-80

Human/duodenum; adenocarcinoma

++

-

++++

++++

++

++++

++++

Vero

African Green Monkey/kidney; normal

+++

+

++++

++

+

++++

++++

RK-13

Rabbit/kidney; normal

++

+

++++

+++

++++

+++

++++

CHO-K1

Hamster Chinese/ovary

++

-

+++

++++

+++

+++

+++

A549

Human/lung; carcinoma

+

-

++

++

+

++

++

PK15

Pig/kidney; normal

++

+

++

++

+

++

+++

NMULI

Mouse/liver; normal

++

-

+++

+++

+

++++

++++

HEK-293 (CRL-1573)

Human/kidney; transformed with adenovirus 5 DNA

+++

-

+++

++

+

++

++

  1. a Virus infection was at a m.o.i of 0.05 pfu per cell. CPE was categorized on the following criteria: no difference from uninfected cells (-); low, < 25% CPE (+); moderate, 25 to 50%CPE (++); high, > 50 to 75% CPE (+++); very high, > 75 to 100% CPE or high level of cell detachment (++++) [25].