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Table 1 Primers used for the construction of pJC2N and site-directed mutagenesis of His6-TGBp2

From: Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus

Primer

Sequences of primers (from 5' to 3')

HM2F

ATCAGAAAGCTT AAGAAGGAGATATACATATGCACCACCACCACCACCAC GACCAGCCTCTTCATCTG

M2R

CTCTTGGGATCC TCCTCAGTGTTTAGCATGGTG

R9A

CCTCTTCATCTGGCCGCA CCACCTGACAACACG

R15A

CCAGACCACCTGACAACACGGCA GCTTACTTAGTATTAGCTATAG

R35A

GTTCCTCTATACACTAACCGCA AATACCCTTCCACACACCGG

R53A

CCGCACGGGGGTGCA TACGTGGACGGCACC

R59A

GTACGTGGACGGCACCGCA GGAATTCTCTACAACAG

R92A

CCTTTTCCTCATCACCGCA AACATTCTCAACCCAGCC

R103A

CCCCCACCACACCTGCA ATCTATGCGCCCC

R114A

CCTCTGCTTGCATTGTCACGCA AATCACCCACCATGCTAAAC

Y54A

CGCACGGGGGTAGGGCA GTGGACGGCACCAAAG

Y63A

GCACCAAAGGAATTCTCGCA AACAGCCCCACCTCCTC

Y70A

CAGCCCCACCTCCTCAGCA CCATCCTCATCTCTC

Y103A

CACCACACCTAGAATCGCA GCGCCCCTCTGCTTG

  1. The underline nucleotide sequence indicates the Hind III or Bam HI restriction site. The italicised bases indicate the six His codons. The sites of Arg or Lys-to-Ala or Tyr-to-Ala substitution are shown in boldface.