ST-246 inhibits membrane precursor biogenesis. BSC-40 cells were infected with 5 PFU per cell of vvF13LGFP in the presence or absence of 10 μM ST-246. Membrane extracts were prepared as described in Materials and Methods and analyzed by immunoblot (left panel). The remaining supernatant was then subjected to immunoprecipitation (right panel). Blots were probed with the indicated antibodies. Left panel, input controls; right panel, immunoprecipitated samples.