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Figure 4 | Virology Journal

Figure 4

From: Vaccinia virus p37 interacts with host proteins associated with LE-derived transport vesicle biogenesis

Figure 4

ST-246 affects the co-immunoprecipitation of membrane-associated p37-GFP, Rab9 and CI-MPR but not p230. BSC-40 cells were infected (A and B) with 5 PFU per cell of vvF13LGFP or mock-infected (C) in the absence (column 1) or presence (column 2) of 10 μM ST-246. The membrane fractions were extracted as described in Materials and Methods, resuspended in hypotonic buffer and immunoprecipitated as indicated. Blots were probed with (A) p230 or Rab9 antibody, (B) GFP or Rab9 antibody, and (C) Rab9 antibody. The top row for each are input controls and are probed as indicated.

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