Skip to main content

Table 1 Characterization of CYT-IVAC hemagglutinin and cytokine content

From: Incorporation of membrane-bound, mammalian-derived immunomodulatory proteins into influenza whole virus vaccines boosts immunogenicity and protection against lethal challenge

Vaccine HA pixel density* HAU/μg of vaccine Total cytokine
(ng/μg vaccine)**
Bioactive cytokine
(pg/μg vaccine)***
PR/8/34 w.t. 5835.4 16 NA NA
PR/8/34 GM-CSF/HA 6407.9 16 185 ± 21 87.3
PR/8/34 IL-2/HA 5562.9 32 4.92 ± 0.3 411
PR/8/34 IL-4/HA 6090.4 32 176 ± 24 456
  1. * Pixel density of HA specific chemiluminescent signal following equal loading of total viral protein
  2. ** Quantitation of virus-incorporated cytokine on protein level based on standard curve of recombinant cytokine (ng of cytokine per ug of vaccine)
  3. *** Quantitation of virus-incorporated cytokine on bioactive level based on standard curve of recombinant cytokine (pg of cytokine per ug of vaccine)