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Figure 6 | Virology Journal

Figure 6

From: Properties and use of novel replication-competent vectors based on Semliki Forest virus

Figure 6

Schematic presentation of the methods for replication-competent vector genome and library construction. (A) Construction of replication-competent vector from restriction fragments of pSFV-PLApa-d1EGFP (Sph I to Apa I) and pHelper96 (Apa I to Spe I). SP6 corresponds to the promoter for SP6 RNA polymerase; bold arrow indicates the ligation process. (B) Construction of library from Sph I/Bam HI restriction fragment of pSFV4-T37/17 and Bam HI-treated product of PCR-based mutagenesis, which contained 21-bp randomized fragment, coding sequence of d1EGFP and SFV4 3'-UTR with poly(A). SP6 corresponds to the promoter for SP6 RNA polymerase and bold arrow indicates the ligation process. Symbols have the same meaning as in Figure 1B. Only the ligation products corresponding to the cDNAs of replication-competent vector (A) or replication-competent vector-based library (B) are shown.

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