Figure 5
From: Properties and use of novel replication-competent vectors based on Semliki Forest virus
Accumulation of viral RNA in BHK-21 cells infected with replication-competent vectors. BHK-21 cells were infected with P1 stocks of SFV4-T21/5-d1EGFP, SFV4-T26/20-d1EGFP, SFV4-T37/17-d1EGFP, SFV4-T99/45-d1EGFP, SFV4-d1EGFP-M26, pSFV4-d1EGFP-M37, pSFV4-d1EGFP-M96, SFV4-EMCV-d1EGFP, SFV4-CR-d1EGFP or SFV4 at an moi of 5. Control cells were mock-infected. Cells were harvested at 12 h post-infection. Total RNA was purified by TRIzol reagent and subjected to agarose electrophoresis and Northern blotting (10 μg total RNA per line). Letters and arrows on the left indicate positions of genomic RNA of vector or virus (A); SG mRNA expressed from native SG promoter (B, except for SFV4 where this mRNA is indicated with an asterisk); SG mRNA expressed from duplicated SG-promoter in middle (C) and terminal (D) vectors. The names of replication-competent vectors are shown above the blot. SFV4 indicates control cells infected with parental SFV4; neg. control indicates RNA from mock-infected cells.