Figure 1
From: Properties and use of novel replication-competent vectors based on Semliki Forest virus
Sequence of SG promoter region and schematic presentation of SFV4-based replication-competent vectors. (A) Sequence of SG region of SFV4 (in positive strand) from position -100 to 60. Arrow indicates start site of SG mRNA, short conserved sequence element of SG promoters of alphaviruses (-35 to -30), unique -1 G residue of SG promoter of SFV4, termination codon for ns-polyprotein reading frame (11 to 13), and initiation codon for structural polyprotein (52 to 54) are shown in color. (B) Schematic presentation of the genomes of replication-competent SFV4-based vectors. "Middle1" represents genomes of middle vectors rescued by use of infectious in vitro transcripts and "Middle2" represents genomes of middle vectors rescued from infectious plasmid by cellular RNA synthesis and splicing machinery. Arrows indicate SG promoters, and UTR-(A)n indicates 3'-UTR of SFV4 with poly(A) sequence. The structure of SG promoters is shown below the drawing, "wt" indicates the native SG promoter of SFV4 and "ns/30" indicates the native SG promoter with truncated downstream region (residues 31 to 51 are deleted). HRP- on structural protein indicates the destabilized capsid hairpin element.