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Figure 3 | Virology Journal

Figure 3

From: The C-terminal sequence of the large hepatitis delta antigen is variable but retains the ability to bind clathrin

Figure 3

An in vitro binding assay of LDAg peptides to HuH-7 cellular clathrin. (A) Expression and purification of GST and the GST fusion proteins with the 13-amino-acid peptides of LDAg in E. coli. Total E. coli protein profiles are in even number lanes and purified proteins are in odd number lanes. The top gel was stained with Coomassie blue and the bottom gel was analyzed for GST. GST fusion proteins from different LDAg genotypes are indicated above the gel. Protein makers are shown to the left of the gel. (B) Western blot analysis for E. coli expressed LDAg peptide binding to CHC from a HuH-7 cell lysate. The top gel was detected for CHC, middle gel for actin, and bottom gel for GST. The GST and GST fusion proteins used for the binding assay are indicated above the gel. Lanes 1 to 5 are total cell lysate and lanes 6–9 are the GST-pull downs.

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