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Figure 2 | Virology Journal

Figure 2

From: The C-terminal sequence of the large hepatitis delta antigen is variable but retains the ability to bind clathrin

Figure 2

An in vivo binding assay of EGFP-LDs to clathrin. HuH-7 cells were transfected by various plasmids, as indicated above the blot, to allow expression of the EGFP-LDs. The total cell lysate and anti-GFP precipitated proteins were analyzed for CHC (2A), GFP (2B), and actin (2C) by Western blotting. NC (lane 1) represents non-transfection HuH-7 cells as a control. Lanes 2 to 5 are total cell lysates and lanes 6 to 9 are the anti-GFP immunoprecipitated proteins.

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