Table 1 Primers and probes used for plasmid construction and real-time PCR

IRESR (Sal I)

5'-GAGTCGACCATGGTTGTGGCCATTATCATCGTG-3'

IGF-IF1 (EcoR I)

5'-CAGAATTCACAATGGGAAAAATCAGCAGTCTTCC-3'

IGF-IF2(Sal I)

5'-ACGTCGACACAATGGGAAAAATCAGCAGTCTTCC-3'

IGF-IR (Bgl II)

5'-CTAGATCTCTACATCCTGTAGTTCTTGTTTCCTG-3'

WF (BamH I)

5'-TCGGATCCGTCCTGTATTAGAGGTCACG-3'

WR (BamH I)

5'-CAGGATCCACTGCTTCTCCGAGGTAATCC-3'

WMF

5'-AACGCGCAGCCGCCACGCCGGGGTTTTACGAG-3'

WMR

5'-TCGTAAAACCCCGGCGTGGCGGCTGCGCGTTC-3'

RCMF

5'-ATCTGCGCAGCCGCCACGCCGGGGTTTTACGAG-3'

RCMR

5'-TCGTAAAACCCCGGCGTGGCGGCTGCGCAGATC-3'

RCMDF

5'-ATCTGCGCAGCCGCCCCGGGGTTTTACGAGATTG-3'

RCMDR

5'-TCGTAAAACCCCGGGGCGGCTGCGCAGATCAGAAG-3'

CMVF

5'-TGGGCGGTAGGCGTGTAC-3'

CMVR

5'-CGATCTGACGGTTCACTAAACG-3'

CMV probe

5'-FAM-TGGGAGGTCTATATAAGCAGAG-MGBNFQ-3'

AAV2F

5'-CAGATTGGCTCGAGGACACTCT-3'

AAV2R

5'-GTGGGCCAGGTTTGAGCTT-3'

AAV2 probe

5'-FAM-TGAAGGAATAAGACAGTGGTA-MGBNFQ-3'