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Figure 1 | Virology Journal

Figure 1

From: Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences

Figure 1

Schematic illustration of AAV helper plasmids. Plasmids pAAV-RC, pAAV-RC/ΔATG and pAAV-RC/ACG contain an AAV2 unrelated heterologous sequence (open boxes) upstream of Rep78/68. pAAV-RC/ΔATG has an ATG deletion at the translation start codon of Rep78/68, while pAAV-RC/ACG has an ATG-to-ACG mutation at the start codon. Plasmid pAAV-RC/p19/ACG contains an extra copy of the 813 bp BamH I fragment of pAAV-RC/ACG. Plasmids pAAV-RC/p5 and pAAV/p5/ACG contain the AAV2 endogenous p5-promoter upstream of Rep78/68 as in wt AAV2. pAAV-RC/p5/ACG has an ATG-to-ACG mutation at the translation start codon of Rep78/68. Shaded boxes represent AAV2 p5-promoter, rep and cap genes. An eleven nucleotide sequence of the p5-promoter is represented by a small shaded box before ATG or ACG in pAAV-RC, pAAV/ΔATG, pAAV/ACG and pAAV-RC/p19/ACG.

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Virology Journal

ISSN: 1743-422X