Epstein-Barr Nuclear Antigen 1 modulates replication of oriP-plasmids by impeding replication and transcription fork migration through the family of repeats

Table 2 Splitting twenty contiguous EBNA1 binding sites into two sets of ten binding sites increases the efficiency of replication as estimated by colony formation.

Replication reporter transfected	Arrangement of EBNA1 binding sites^a	Colonies per 10⁵ live, transfected cells plated^b
AGP73	FR(10), DS	4390 ± 311.1^c
AGP74	FR(20), DS	1296 ± 106.1^c
AGP212	FR(10), DS, FR(10)	10048 ± 371.7
AGP213	FR(10), FR(10), DS)	6132 ± 180.2

^a The arrangement of EBNA1 binding sites in FR is schematically depicted in Figure 3.
^b puromycin resistant colonies present 18 days post-transfection that are 2 mm and larger in size.
^c values are taken from Table 1, and shown here for convenience,

ISSN: 1743-422X