Epstein-Barr Nuclear Antigen 1 modulates replication of oriP-plasmids by impeding replication and transcription fork migration through the family of repeats

Table 1 A greater than wild-type number of EBNA1 binding sites in the family of repeats causes a decrease in the number of puromycin resistant colonies obtained in colony formation assays.

Replication reporter transfected	Number of EBNA1 binding sites in FR^a	Colonies per 10⁵ live, transfected cells plated^b
AGP83	0	2 ± 1.8
AGP73	10	4390 ± 311.1
AGP74	20	1296 ± 106.1
2380	20^c	1230 ± 28.3
AGP81	40	78 ± 14.2
AGP82	80	3 ± 1.7

^a plasmids contain DS, EBV sequences between FR and DS, and a synthetic FR containing the indicated number of EBNA1 binding sites.
^b puromycin resistant colonies present 18 days post-transfection that are 2 mm and larger in size.
^c plasmid 2380 contains wild-type FR from the B95-8 strain of EBV.

ISSN: 1743-422X