Figure 5From: Engineering the vaccinia virus L1 protein for increased neutralizing antibody response after DNA immunizationEffects of signal peptide and presence or absence of transmembrane domain on VACV neutralizing antibodies. Mice (n = 5) were immunized at 0 time and weeks 2, 4 and 6 with plasmids using a gene gun. Mice were bled at 2 weeks after each of the first three immunizations and 3 weeks after the last. Neutralizing activity was determined by flow cytometry. Arrows point to days of immunizationBack to article page