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Figure 1 | Virology Journal

Figure 1

From: Engineering the vaccinia virus L1 protein for increased neutralizing antibody response after DNA immunization

Figure 1

Expression of modified L1 proteins in BS-C-1 cells determined by Western blotting. Cells were harvested at 24 h after transfection, lysed, denatured with (A) or without (B) reducing agent, and subjected to SDS-PAGE. The proteins were transferred to a membrane and probed with polyclonal antibody to L1 and detected by chemiluminescence. Lanes: 1, pL1; 2, pL1op; 3, psL1op; 4, empty vector; 5, VACV-infected lysate. The position of a 28-kDa marker protein is shown on the left.

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