| 1-992 | aa365-545 | aa365-545 m2 |
---|
SUMO-3
| ++ | +++ | - |
RBP-J-κ
| +++ | - | - |
Gadd34
| ++ | +++ | - |
- Focused yeast two-hybrid assays testing the interaction of full-length EBNA3C, EBNA3C aa365-545 Yeast strain AH109 were transformed in parallel with pAS1-EBNA3C (1-992), pAS-WT EBNA3C (aa365-545) or with the SIM (M2) mutant (aa365-545, 509DVIEVID515→509AVIAVIA515) and tested for interaction with pACT-Gadd34aa395-674. Colonies were sampled using a filter lift assay and interaction confirmed using a LacZ assay. Results of a 1 hour LacZ assay, scored semi-quantitatively, are shown.