Skip to main content

Table 1 Focused yeast two-hybrid assays

From: EBNA3C interacts with Gadd34 and counteracts the unfolded protein response

 

1-992

aa365-545

aa365-545 m2

SUMO-3

++

+++

-

RBP-J-κ

+++

-

-

Gadd34

++

+++

-

  1. Focused yeast two-hybrid assays testing the interaction of full-length EBNA3C, EBNA3C aa365-545 Yeast strain AH109 were transformed in parallel with pAS1-EBNA3C (1-992), pAS-WT EBNA3C (aa365-545) or with the SIM (M2) mutant (aa365-545, 509DVIEVID515509AVIAVIA515) and tested for interaction with pACT-Gadd34aa395-674. Colonies were sampled using a filter lift assay and interaction confirmed using a LacZ assay. Results of a 1 hour LacZ assay, scored semi-quantitatively, are shown.