Figure 1
From: EBNA3C interacts with Gadd34 and counteracts the unfolded protein response
Gadd34 aa 483-610 are required for EBNA3C association. a) 293-T cells were seeded in 6-well plates resulting in 70% confluence by day 2, and transfected with 0.5 μg of either psg5-EBNA3C alone (lane 1), psg5-Flag-Gadd34 (full-length, aa1-674, lane 2), or both EBNA3C and Flag-Gadd34 (1-674) (lane 3). In lanes 4-7 a combination of EBNA3C and the indicated Gadd34 mutants were transfected. Total DNA was 1.0 μg for each transfection. 18 hours post-transfection, cells were lysed in isotonic buffer containing 0.5% NP-40. Gadd34 or Gadd34 mutant proteins were immune precipitated with anti-flag affinity beads (M2-agarose), and precipitating proteins western blotted with α-EBNA3C antibody (A10) (top panel) or Flag antibody (bottom panel). In lane 7, 3% of starting lysates prior to immune precipitation (input) from the well transfected with psg5-EBNA3C alone (top panel), or psg5-Flag-Gadd34 alone (bottom panel) is shown.