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Figure 2 | Virology Journal

Figure 2

From: Mutagenesis of the transmembrane domain of the SARS coronavirus spike glycoprotein: refinement of the requirements for SARS coronavirus cell entry

Figure 2

Capacity of S proteins with mutations in the aromatic domain to mediate entry of SARSpp into VeroE6R cells. A) Titers of the mutant S containing pseudoparticles are shown as a percentage of wild type S containing pseudoparticles. B) Incorporation of S proteins in pseudoparticles. Equal amounts of [35S]-labeled particles, based on RT activity, were subjected to immunoprecipitation, using an S-specific antibody. The precipitates were analyzed using SDS-PAGE and subsequent autoradiography. C) Trimers of S protein were shown by running the precipitates of B on a non reducing gel, without boiling of the samples.

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Virology Journal

ISSN: 1743-422X