Disorder predictions of caliciviral VPgs. Five predictors were used: PONDR® VLXT, FoldIndex©, DISOPRED2, VSL2, IUPred. The location of predicted disordered (in the order provided by the above-listed predictors) was schematically represented by lines along the VPg sequence. Numbering represents the VPg length. The consensus predicted α-helices and β-strands are indicated. The conserved tyrosine residue (Y) involved in VPg urydylylation is indicated. RHDV Rabbit hemorrhabic disease virus (Lagovirus), VESV Vesicular exanthema of swine virus (Vesivirus), SV Man Sapporo virus Manchester virus (Sapovirus) and NV Norwalk virus (Norovirus).