Disorder predictions of sobemoviral VPgs. Five predictors were used: PONDR® VLXT, FoldIndex©, DISOPRED2, VSL2, IUPred. The location of predicted disordered regions (in the order provided by the above-listed predictors) was schematically represented by lines along the VPg sequence. Numbering indicates the VPg length. The consensus predicted α-helices and β-strands are indicated. The sites involved in RYMV virulence (*) are indicated. The VPgs experimentally demonstrated to be disordered are shaded. RYMV Rice yellow mottle virus, CoMV Cocksfoot mottle virus, RGMoV Ryegrass mottle virus, SBMV Southern bean mosaic virus, SCPMV Southern cowpea mosaic virus, SeMV Sesbania mottle virus.