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Figure 2 | Virology Journal

Figure 2

From: Increased production of viral proteins by a 3'-LTR-deleted infectious clone of human T-cell leukemia virus type 1

Figure 2

Various molecular clones with deletions of the 3' LTR of different lengths and the corresponding viral expression patterns. (A) Diagram of the molecular clones showing the deletion series of the 3' LTR and the deletion of the reverse TATA box of the 3' LTR. (B) The amount of p19 gag in the pooled concentrated (100-fold) medium from 293T cells transfected with the complete molecular clone pFL-MT2 or with clones containing deletions of the 3' LTR of different lengths and the deleted reverse TATA box 2-3 days after transfection. The results of RT-PCR detection of tax mRNA in cells transfected with the various clones are shown on the left side.

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