Figure 5

TaqMan Real-Time qRT-PCR for quantification of negative and positive-strand DWV RNA in tissues of symptomatic and asymptomatic bees. The hemolymph, gut, wings, legs, head, thorax and abdomen were individually dissected out from symptomatic and asymptomatic bees and subjected to TaqMan real-time qRT-PCR coupled with biotinylated primer and magnetic beads purification. The virus titer of negative-strand (A) and positive-strand (B) DWV of each sample was quantified with the standard curve and expressed as copy numbers (log).