Figure 4
Amplification plot and standard curve of TaqMan quantitative RT-PCR (qRT-PCR) incorporated with biotinylated primer and magnetic beads purification. In vitro transcribed DWV RNA was serially diluted and subjected to RT-PCR to assess the sensitivity of the assay. A) Amplification plots were generated by using seven serial dilutions of the RNA, ranging from 103 pg to 1 fg per reaction as the template for the qRT-PCR assays. The amplification plot shows the fluorescence (dR) plotted against the cycle number for the standard dilution series of DWV. B) Standard curves were generated by plotting the observed CT value (Y axis) against the initial quantities of 10-fold serial diluted RNA. CT values are the average of three repetitions.