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Figure 1 | Virology Journal

Figure 1

From: Molecular approaches to the analysis of deformed wing virus replication and pathogenesis in the honey bee, Apis mellifera

Figure 1

Conventional RT-PCR for strand-specific detection of DWV RNA. Total RNAs extracted from DWV-infected bees. Both negative and positive-strands of DWV RNA were specifically amplified by conventional RT-PCR using DWV-specific forward (line 2) and reverse primer (line 3), respectively, for initial reverse transcription. RT-PCR amplification, was also conducted without inclusion of any primers for reverse transcription (line 4). Negative controls containing no template (line 5) and no reverse transcriptase (line 6) yielded no products. The 100-bp ladder was loaded into lane 1. The arrow on the right indicates the expected 702 bp RT-PCR products.

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