Interaction between Gas8 and β-actin. (A)SDS-PAGE analysis of proteins that co-IPed with Gas8. 293FT cells were transfected with pCMV-Tag 2B-GAS8 or pCMV-Tag 2B, and cell extracts were immunoprecipitated with anti-FLAG antibody. The gels were Coomassie stained. 1: pCMV-Tag 2B/293FT, 2: pCMV-Tag 2B-GAS8/293FT. Arrows indicate proteins that co-IPed with Gas8. (B) Gas8 co-localizes with β-actin at the plasma membrane. CV-1 cells were transfected with pDsRed-GAS8. After 24 h, the cells were fixed and probed with an antibody against β-actin. The merged image shows co-localization of Gas8 (red) and β-actin (green) at the plasma membrane. (C) Interaction of Gas8 and β-actin proteins in vitro. 293FT cells were transfected with pCMV-Tag 2B-GAS8 (FLAG-Gas8) or pCMV-Tag 2B (FLAG). Soluble cell lysates were IPed with anti-FLAG or anti-β-actin antibody and probed with anti-FLAG (left) or anti-β-actin antibody (right).