Table 1 Nucleotide sequences of the oligonucleotides used for PCR amplification and sequencing
Primer set | Primer name | Nucleotide sequence | Position |
|---|---|---|---|
1 | SP1F | GTGATCGGTGATGGCTAATTGCCG | 1-14 |
| Â | SP1R | TGGAGATGGTATCTGTCAGTGTG | 645-667 |
2 | SP2F | GGCAGTACATTTGTGAGGGGTG | 543-564 |
| Â | SP2R | CCTGTTCTGCTTTATCACCTCC | 1170-1191 |
3 | SP3F | GACGGTGGCTGCCATTAAAGCTG | 1063-1085 |
| Â | SP3R | GCAGTGTAGCCGCGTACTGAGC | 1833-1854 |
4 | SP4F | ATTGACGTGACAGCCCCCAC | 1733-1752 |
| Â | SP4R | TGTGGTTCTTGACTGGTGAG | 2335-2354 |
5 | SP5F | TGGTGGAGGCCTGTTCAGAGC | 2223-2243 |
| Â | SP5R | CCAAGTTGTGGGCTGTCAACAC | 2757-2778 |
6 | SP6F | CAGAGTCCTCCTGGTGGACATTC | 2680-2702 |
| Â | SP6R | ATTACCAAGCGCAACGCTAGGC | 3340-3361 |
7 | SP7F | CATGTGGCCAACATGTGTG | 3243-3261 |
| Â | SP7R | TGATTTGGTCAAGGTAGCC | 3873-3891 |
8 | SP8F | CCTTCTACAACACCAAATGATTGCC | 3768-3792 |
| Â | SP8R | AGGCCAGGATGTCAACACTGGCAC | 4371-4394 |
9 | SP9F | ATGTATGGATAGCCCTCAGATTG | 4261-4283 |
| Â | SP9R | GTCCACATCAACGGCCGCCGGCTCG | 4890-4914 |
10 | SP10F | AGCCAACAGACACTCCTGTGTTCC | 4760-4783 |
| Â | SP10R | CATGCCAGACCCTGATATTATCACC | 5468-5492 |
11 | SP11F | ACCTACACCAATGTCACCTGGAC | 5328-5350 |
| Â | SP11R | GTGCCACACCTACTATGACCACAG | 5890-5913 |
12 | SP12F | TCAAGCCTCCAAACCAAGCC | 5784-5803 |
| Â | SP12R | TGGCGGTCCATAAATGAGGTG | 6395-6415 |
13 | SP13F | TATGCAGCTTTGGCAATTCCC | 6291-6311 |
| Â | SP13R | TTGATCTTTAGCAACTGTATCTG | 6892-6915 |
14 | SP14F | TTGGATTGCAGGAGCAATGCAGG | 6777-6799 |
| Â | SP14R | TGTAAGGTTCGGTACGCGTAACC | 7280-7303 |
15 | SP15F1 | TCAATTGGCTGGGTCACGTGAAG | 7027-7049 |
| Â | SP15F2 | CAAACACCTTTGGTCCACCAAGG | 7070-7092 |