Figure 4
From: Hepatitis C virus core protein induces apoptosis-like caspase independent cell death
Influence of HCV- core protein on the receptor-mediated and the mitochondrial apoptosis pathway studied in different assays: 1 × 105 UC cells/ml were cultured for 48 h in the presence or absence of Tet to induce specific protein expression. Apoptotic stimuli were added during the last 24 h: mitomycin C (50 μg/ml), etoposide (400 ng/ml), TRAIL (40 ng/ml) and anti-CD95 antibody (100 ng/ml). A: Induction of apoptosis was assessed by flow cytometric analysis of propidium iodide staining of hypodiploid apoptotic nuclei. The mean values and standard deviation of triplicate cultures are shown. B: Apoptosis was visualized by the externalization of PS which was stained with Annexin V and C: viability of the cells was measured by staining of the cells with PI and subsequent detection by flow cytometry. Given are means of duplicates. D: Metabolic activity of the UC cells was determined by the MTS test. Optical density was measured in an ELISA reader after incubation of the cells with MTS for 4 h and suspension of crystals. Given are mean and standard deviation of triplicates.