The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

Table 1 The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

	Complete or divided promoters in CAT reporter plasmids for transfection
Transfected CEFs	Mock control pCAT-Basic	pP_GA(pp38) -CAT	pdP_GA(pp38) -CAT	pP_GA(1.8 kb) -CAT	pdP_GA(1.8 kb) -CAT	pP_CVI(pp38) -CAT	pP_CVI(1.8 kb) -CAT
Uninfected	3 ± 0 (3~3, n = 4)	4 ± 0 (4~4, n = 4)	4 ± 0 (4~4, n = 3)	4 ± 0 (4~4, n = 4)	4 ± 0 (4~4, n = 3)	4 ± 0 (4~4, n = 4)	4 ± 0 (4~4, n = 4)
rMd5-infected	3 ± 0 (3~3, n = 4)	52 ± 6.28 (41~60, n = 5)	34 ± 3.1 (29~39, n = 4)	781 ± 55.1 (704~842, n = 4)	19 ± 2.1 (16~23, n = 5)	54 ± 4.01 (47~68, n = 5)	635 ± 27.4 (587~700, n = 5)

The CAT expression levels were presented in concentrations (pg/mL) of the lysates prepared as in Materials and Methods. The statistics analysis was made between each pairs. For each sample, numberical figures represent following data: mean ± S.E., ranges and repeated numbers transfection assay with a given reporter plasmid. CAT activity was compared for each pairs related each factors such as CEF infection status, the complete or divided promoter and the direction of the bi-directional promoter.