Figure 1From: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease virusesComparisons of CAT expression levels in uninfected CEF or rMd5-infected CEF cells transfected with plasmids pP GA (pp38)-CAT and pP GA (1.8 kb)-CAT. Transfected cells were harvested and lysed by 3 repeats of freeze and thraw The lysed samples were analyzed for CAT activity in 96-well plate of Roche's CAT ELISA kit. Each value represents the average of at least four independent transfections and significant differences were analyzed by student's test. *, p < 0.05, compared with the pPGA(pp38)-CAT.Back to article page