Table 1 Primers for amplification of the glycoprotein B and UL33 genes
Primer-set | Name of primer | PCR round | Sequence 5'-3' | Product length$ |
|---|---|---|---|---|
CMV-gBp1 | 4007s | 1 | GGTGACGTGGTTGACAT(n/i)TC(n/i)CC | 800 |
4007as | GCAATCGGTTTGTTGTA(n/i)AT(n/i)GC | |||
4125s | 2 | GCGGACTCGGTGAT(b/i)TC(n/i)TGGGA | 550 | |
4125as | GCTGACGGGTTGATCTTGCT(r/i)AG | |||
CMV-gBp2 | 4524s | 1 | GGAATCCAGGAT(y/i)TGGT(r/i)CCT | 1790 |
4524as | TGTCCTCACCCAG(y/i)TG(n/i)CC(r/i)TA | |||
4525s | 2 | CTGGTGCCTGG(y/i)AG(y/i)CTGCG | 1590 | |
4525as | CGGTTTGTTGTA(r/i)ATGGC(n/i)GA | |||
CMV-UL33 | 4068s | 1 | GGAAACGCAGCTC(n/i)CG(r/i)TA(r/i)TA | 2000 |
4068as | TGTCCTGGTGCTCGCA(r/i)TA(r/i)TG | |||
4069s | 2 | GCGCCGCTG(n/i)AG(n/i)CC(r/i)AT | 1700 | |
4069as | GGAGAAGTC(y/i)CGGGT(r/i)GT(n/i)AT |