Skip to main content
Figure 2 | Virology Journal

Figure 2

From: High genotypic diversity and a novel variant of human cytomegalovirus revealed by combined UL33/UL55 genotyping with broad-range PCR

Figure 2

Binding sites of LNA-oligonucleotides in the gB/ UL55 and UL33 genes of HCMV. The binding sites of the LNA used in this study for specific inhibition of HCMV gB/ UL55 (A-D) and UL33 (E-G) genotype amplification are boxed and aligned with the corresponding regions of the other three gB genotypes. The LNA- bases and sequences identical to the LNA sequence are highlighted in yellow/green. On top of alignments A-D, the positions in the gB genes of gB genotype CLS-1 [M60929] (A), gB genotype CLS-2 (strain AD169)(B), gB genotype CLS-3 [M85228](C) and gB genotype CLS-4 [M60926] (D) are shown. LNA-gB-1 and LNA-gB-4 bind to the sense strand, LNA-gB-2 and LNA-gB-3 bind to the antisense strand. On top of the alignments E-G, the positions in the genes of UL33 genotype 1 (strain Merlin) (E), UL33 genotype 3 (strain Toledo)(F) and UL33 genotype 4 (strain AD169)(G) are shown. LNA-UL33-1/2 binds to the antisense strand, LNA-UL33-3 and LNA-UL33-4 to the sense strand.

Back to article page