Figure 1

Amplification of HCMV gB/ UL55 and UL33 using LNA-supplemented consensus PCR. Schematic diagram of the analysis strategy. Nested PCR is performed with deg/dI primers (black triangles). The primers CMV-gBp1 target the CLS of the gB gene, and the primers CMV-gBp2 target the gB gene from the N-terminus to the CLS (A). The complete UL33 gene is amplified by the CMV-UL33 primers (B). The binding regions of the LNA-oligonucleotides are present in the amplified sequences of both the first and the second PCR round, and represented by short thick lines (the position varies slightly for each LNA). The targeted HCMV genotypes are indicated.