The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques

Table 3 Epitope mapping of MneRV2 ORF59 using N- and C-terminal truncation mutants.

MneRV2 ORF59 truncation¹	N-terminal residue²	C-terminal residue²	Macaque serum³
			DBL2	J00072	K99344
T1	1	101	+	+	+
T2	1	205	+	+	+
T3	1	291	+	+	+
T4	1	360	+	+	+
T5	299	394	-	-	-
T6	354	394	-	-	-

¹orf59 gene truncations were prepared using primers listed in Table 1 and truncated proteins were expressed in bacteria as described in Materials and Methods.
²Amino acid residue number from the sequence of MneRV2 ORF59 (Fig. 1)
³Serum (1:1000 dilution) from SIV-negative rhesus macaque (DBL2) and SIV-positive pig-tailed macaques (J00072 and K99344) were reacted with MneRV2 ORF59 truncation mutants in Western blots as described in Figure 4 and the Materials and Methods.

ISSN: 1743-422X