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Figure 8 | Virology Journal

Figure 8

From: The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques

Figure 8

RRV infection of RPFF and Vero cells is permissive with replication of viral genomes and production of infectious virions. Near confluent cultures of RPFF (solid lines) and Vero cells (dotted lines) were infected with RRV and incubated for various times. Cell-free (triangles) and cell-associated (squares) RRV DNA was quantitated by TaqMan qPCR from culture medium and cell pellets, respectively. The 0-time point reflects the amount of input virus that bound to the cell monolayer during the adsorption period. For the longer time points, the spent culture medium was collected during the course of the incubation, combined and PEG precipitated before analysis.

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Virology Journal

ISSN: 1743-422X