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Figure 7 | Virology Journal

Figure 7

From: The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques

Figure 7

ORF59 is a marker for RRV infection and provides evidence for efficient virus transmission in local environments. Subconfluent cultures of RPFF cells were infected with RRV (~MOI 0.01) for 2 hours. Input virus was removed and cells were washed, incubated for various times, fixed and reacted with the 425 anti-RV2 ORF59 antiserum. ORF59 expression was visualized by confocal immunofluorescence microscopy and fluorescent nuclei were manually counted. Single cell and multi-cell foci of infection were differentiated (see visual examples in Fig. 6A, C). A) singe cell foci - dotted bar; multi-cell foci - solid bar B) cells per multi-cell foci - dashed bar; cells per all foci - dotted bar.

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