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Figure 5 | Virology Journal

Figure 5

From: The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques

Figure 5

The 425 rabbit anti-RV2 ORF59 antiserum specifically recognizes the ORF59 proteins from the RV2 rhadinoviruses of three macaque species. Recombinant C-terminal polypeptides that were highly conserved between RRV and MneRV2 (see underlined antigenic sequences in Fig. 1) were expressed in bacteria with a 6XHis tag, purified and used to immunize a rabbit (425). Full length recombinant ORF59 proteins from RRV (Lane 1), RFHVMm (Lane 2), MneRV2 (Lane 3), RFHVMn (Lane 4), PtrRV2 (Lane 5), PtrRV1 (Lane 6) and KSHV (Lane 7) were expressed as glutathione synthase tranferase (GST) fusions, analyzed by SDS-PAGE and probed using either A) 425 rabbit anti-RV2 ORF59 antiserum, or B) anti-GST antiserum, as described in the Materials and Methods.

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Virology Journal

ISSN: 1743-422X