Hepatitis A virus (HAV) packaging size limit

Table 2 Insertions in the HAV 2A-2B junction or 5'NTR

Construct	Insert	Site	Size (nt) *	Infectivity^&
pHAVvec9	polylinker	2A-2B	66	+
pHAVvec9-Bsd	Bsd	2A-2B	396	+
pHAVvec9-GFP-Bsd	GFP-Bsd	2A-2B	1098	-
pHAVvec9-Hyg	Hygromycin	2A-2B	1032	-
pHAV-IRES	EMCV IRES	5'NTR	606	+
pHAVvec9-Bsd-IRES	Bsd	2A-2B	396	-
	EMCV IRES	5'NTR	606

* pHAVvec9-Bsd, pHAVvec9-GFP-Bsd, and pHAVvec9-Hyg, and pHAVvec9-Bsd-IRES contain additional 60-nt corresponding to the polylinker inserted at the 2A-2B junction.
^&Infectivity (+) or lack of infectivity (-) of the T7 polymerase in vitro synthesized transcripts from the plasmid constructs was assessed in FRhK4 cells by IF analysis.