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Table 1 PCR Primers

From: Hepatitis A virus (HAV) packaging size limit

Name*

PCR Fragment

Oligonucleotide Sequence&

Bsd forward (SalI)

Bsd

5'-GTCGACGTCGACCAGGCCAAGCCTTTGTCTCAAGAA-3'

Bsd reverse (KpnI)

Bsd

5'-CGGTTAGGTACCGCCCTCCCACACATAACCAGAGGG-3'

GFP forward (SalI)

GFP-Bsd

5'-GTCGACGTCGACGCCTCCAAAGGAGAAGAACTTTTC-3'

Hyg forward (SalI)

Hyg

5'-GTCGACGTCGACAAAAAGCCTGAACTCACCGCGACG-3'

Hyg reverse (KpnI)

Hyg

5'-CGGTTAGGTACCGTTAGCCTCCCCCATCTCCCGATC-3'

EMVC IRES forward (XbaI)

EMCV IRES

5'-TTAGTCTAGATGGTACCTACGTACCGCGGAATTCCGCCCCTCTCCCTAACGTTACTGGCCGAA-3'

EMVC IRES reverse (XbaI)

EMCV IRES

5'-TTTCTAGACATGTTCATATTATCATCGTGTTTTTCAA-3'

  1. *Restriction enzymes in brackets cut the PCR fragment amplified with the oligonucleotides.
  2. &The restriction site in each oligonucleotide is underlined.