Figure 1

Activation of antiviral response by RIGI during HPIV3 infection. (a) Western blot analysis (with anti-FLAG antibody) of RIGI expression in 293 cells transfected with pcDNA and RIGI (FLAG tagged) plasmids. (b) Activation of IRF3-luciferase and NF-κB-luciferase in mock and HPIV3 infected (at 8 h post-infection) 293 cells transfected with either pcDNA or RIGI. The luciferase assay results are presented as mean ± standard deviation from three independent experiments. (c) RT-PCR analysis of TNF and IFN-β expression (at 4 h and 8 h post-infection) in mock and HPIV3 infected 293 cells transfected with either pcDNA or RIGI. GAPDH served as a loading control. NS; non-specific. (d) RT-PCR analysis of ISG-15 expression (at 2 h, 4 h and 8 h post-infection) in mock and HPIV3 infected 293 cells transfected with either pcDNA or RIGI