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Table 2 Validation of microarray results by real-time PCRa

From: Gene expression in primate liver during viral hemorrhagic fever

Gene Symbol

WE vs uninfected

WE vs no disease

(GenBank ID)

Pre-viremic

Viremic Stage

Pre-viremic

Viremic stage

ACACB (AI057637)

-3.58/-5

-4.38/-5

-2.16/-2

-2.64/-2

ACVR1 (NM_001105)

10.85/15

7.62/12

7.46/11

5.24/10

ARG2 (U75667)

1.02/2

1.04/1

-12.13/-9

-11.88/-5

C1S (BC007010)

53.45/25

50.21/22

6.73/4.2

6.32/3.7

CYP26A1(NM_000783)

9.85/13

2.11/1.2

10.56/14

2.27/2.9

FGB (BG545288)

49.87/32

45.57/29

3.36/2.4

3.07/2.0

FST (NM_013409)

-1.67/1.8

1.09/1.3

-16.8/-19

-9.25/-16

GOT1 (BC000498)

3.92/3.0

2.0/1.6

-6.54/-13

-12.82/-18

HBA1/HBA2 (V00489)

2.79/2

1.2/3.1

-12.91/-11

-30.06/-25

HSPB1 (NM_001540)

28.64/22

36.76/43

9.38/7.2

12.04/9.7

IFI44 (NM_006417)

19.43/14

198.1/166

3.71/4.0

37.79/21

INHBA (M13436)

1.71/1.9

-1.13/1.3

11.16/15

5.78/8.7

PPARGC1A (NM_013261)

-1.22/1.0

3.01/1.9

-21.41/-9

-5.82/-3

PPP1R3C (N26005)

25.28/32

9.99/12

156.5/87

61.82/45

SDS (NM_006843)

-1.97/1.7

-2.07/1.2

-32.22/-28

-33.82/-29

TGFBR1 (AA604375)

4.29/4.8

3.1/2.5

1.33/1.6

-1.04/1.5

PRKCSH (AI815793)

<2/-b

<2/-

<2/-

<2/-

  1. aThe numbers represent N-folds from Affychip data (most in Figure 4, all in Additional file 8) and N-fold by real-time PCR (Naffy/NPCR). N-folds by real-time PCR make use of liver RNA from uninfected, pre-viremic LCMV-WE-infected, viremic LCMV-WE-infected, and infected-but-not-diseased monkeys.
  2. bPRKCSH is the gene symbol for protein kinase C substrate 80 K-H to which the PCR data are normalized. It is expressed in liver and has a less than 0.04 standard deviation over all samples used for this liver transcriptome analysis. (-) means it is the reference value.
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