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Table 2 Validation of microarray results by real-time PCRa

From: Gene expression in primate liver during viral hemorrhagic fever

Gene Symbol WE vs uninfected WE vs no disease
(GenBank ID) Pre-viremic Viremic Stage Pre-viremic Viremic stage
ACACB (AI057637) -3.58/-5 -4.38/-5 -2.16/-2 -2.64/-2
ACVR1 (NM_001105) 10.85/15 7.62/12 7.46/11 5.24/10
ARG2 (U75667) 1.02/2 1.04/1 -12.13/-9 -11.88/-5
C1S (BC007010) 53.45/25 50.21/22 6.73/4.2 6.32/3.7
CYP26A1(NM_000783) 9.85/13 2.11/1.2 10.56/14 2.27/2.9
FGB (BG545288) 49.87/32 45.57/29 3.36/2.4 3.07/2.0
FST (NM_013409) -1.67/1.8 1.09/1.3 -16.8/-19 -9.25/-16
GOT1 (BC000498) 3.92/3.0 2.0/1.6 -6.54/-13 -12.82/-18
HBA1/HBA2 (V00489) 2.79/2 1.2/3.1 -12.91/-11 -30.06/-25
HSPB1 (NM_001540) 28.64/22 36.76/43 9.38/7.2 12.04/9.7
IFI44 (NM_006417) 19.43/14 198.1/166 3.71/4.0 37.79/21
INHBA (M13436) 1.71/1.9 -1.13/1.3 11.16/15 5.78/8.7
PPARGC1A (NM_013261) -1.22/1.0 3.01/1.9 -21.41/-9 -5.82/-3
PPP1R3C (N26005) 25.28/32 9.99/12 156.5/87 61.82/45
SDS (NM_006843) -1.97/1.7 -2.07/1.2 -32.22/-28 -33.82/-29
TGFBR1 (AA604375) 4.29/4.8 3.1/2.5 1.33/1.6 -1.04/1.5
PRKCSH (AI815793) <2/-b <2/- <2/- <2/-
  1. aThe numbers represent N-folds from Affychip data (most in Figure 4, all in Additional file 8) and N-fold by real-time PCR (Naffy/NPCR). N-folds by real-time PCR make use of liver RNA from uninfected, pre-viremic LCMV-WE-infected, viremic LCMV-WE-infected, and infected-but-not-diseased monkeys.
  2. bPRKCSH is the gene symbol for protein kinase C substrate 80 K-H to which the PCR data are normalized. It is expressed in liver and has a less than 0.04 standard deviation over all samples used for this liver transcriptome analysis. (-) means it is the reference value.