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Table 1 Macaque liver tissues used for transcriptome analyses

From: Gene expression in primate liver during viral hemorrhagic fever

Liver samples AST/ALTd Glucosee Triglyceridesf Virus titerg
Uninfected controls     
   Rh Ctrl7 38/65 74 65 <102
   Rh Ctrl8 24/53 79 61 <102
   Rh Ctrl9 47/71 87 77 <102
LCMV-WE1-Pre-viremic     
   Rh 1WE (day 1)a 44/61 53 27 <102
   Rh 2WE (day 1) 63/54 58 30 <102
   Rh 3WE (day 2) 59/37 60 18 <102
   Rh 5WE (day 3) 27/71 62 37 <102
LCMV-WE2-Viremic b     
   Rh 6WE (day 4) 96/137 71 33 <102
   Rh 7WE (day 4) 24/38 67 18 <102
   Rh 8WE (day 6) 26/56 54 32 1.4 × 10 4
   Rh 9WE (day 6) 125/82 70 31 5.0 × 10 3
   Rh 10WE (day 7) 190/325 66 30 1.5 × 10 6
LCMV-WE3 Terminal     
   Rh 11WE (day 11) 642/587 30 235 5.5 × 10 5
   Rh 12WE (day 12) 1504/681 40 1402 1.0 × 10 6
LCMV-not diseased c     
   Rh 3ARM (day 3) 45/74 70 75 <102
   Rh 5ARM (day 5) 18/40 78 62 <102
   Rh 1ARM/WE-1 52/24 65 79 <102
   Rh 1ARM/WE-5 Not done Not done ND <102
   Rh 2ARM/WE-6 56/34 63 80 <102
   Rh 2ARM/WE-2 Not done Not done ND <102
  1. a (day 1) is the day of necropsy after infection. Each sample is from the liver of a different rhesus macaque.
  2. b Pre-viremic means liver was harvested day 1, day 2 or day 3 after infection; Viremic means liver was harvested day 4 to day 7, and Terminal means liver was harvested day 11 or 12 during the terminal stage of disease. ND = Not done.
  3. c Animals that were LCMV-infected but not diseased were either infected with LCMV-ARM and sacrificed day 3 or day 5 after infection, or they were infected with LCMV-ARM and LCMV-WE (103 pfu each virus) and sacrificed on day 30 after infection.
  4. d AST/ALT means the ratio of plasma levels of aspartate aminotransferase to alanyl aminotransferase in international units per liter (IU/L). Normal ranges are AST/ALT = 18–82/22–87 IU/L. Values outside the reference range are bolded.
  5. e Serum glucose levels are in mg/dL with a reference range of 55–80 mg/dL (Animal Diagnostics Laboratory, Baltimore, MD.)
  6. f Serum triglyceride levels have a reference range of 50–82 mg/dL. Other parameters derived from blood analysis include red blood cells, hemoglobin levels and cholesterol levels which were all within normal ranges for all samples.
  7. g Virus was titered on Vero cell monolayers as described [2], and given as plaque forming units per gram of liver tissue. Samples in the viremic group that had undetectable titers (<102 pfu/gm) were positive for virus nucleic acid by RT-PCR and virus co-cultivation as described previously [3].