Skip to main content
Figure 3 | Virology Journal

Figure 3

From: An HPV 16 L1-based chimeric human papilloma virus-like particles containing a string of epitopes produced in plants is able to elicit humoral and cytotoxic T-cell activity in mice

Figure 3

Analysis of soluble protein extracts by immunodetection. Total proteins were extracted from tomato fruit from the T1 generation and 40 μg were loaded per lane, separated by 12% SDS PAGE, transferred onto a PDVF membrane, incubated with a mouse monoclonal anti-L1 antibody and the bands detected by chemiluminescence as described in the text. Top panel, transgenic lines L1. Bottom panel, transgenic lines L1-E6/E7. mwm molecular weight markers, wt non-transformed tomato plant. The arrows indicate the bands present in transformed plants but not in non-transformed plants. Additional bands detected by the antibody can be seen in the bottom panel.

Back to article page