Figure 3From: An HPV 16 L1-based chimeric human papilloma virus-like particles containing a string of epitopes produced in plants is able to elicit humoral and cytotoxic T-cell activity in miceAnalysis of soluble protein extracts by immunodetection. Total proteins were extracted from tomato fruit from the T1 generation and 40 μg were loaded per lane, separated by 12% SDS PAGE, transferred onto a PDVF membrane, incubated with a mouse monoclonal anti-L1 antibody and the bands detected by chemiluminescence as described in the text. Top panel, transgenic lines L1. Bottom panel, transgenic lines L1-E6/E7. mwm molecular weight markers, wt non-transformed tomato plant. The arrows indicate the bands present in transformed plants but not in non-transformed plants. Additional bands detected by the antibody can be seen in the bottom panel.Back to article page