Comparative plaque assays for MNV-1 detection. Cells were infected with same preparation of diluted MNV-1, maintaining a consistent MOI. Following a one-hour adsorption period, infected cultures were covered with a 1.5% methylcellulose overlay medium. Cells were then stained with a crystal violet-formalin solution at day 3 post infection and titers were calculated. A. BV-2 plaque assay; B. RAW 264.7 plaque assay; and C. TIB plaque assay.