RT-PCR. Confirmatory detection of MNV-1-infection of test cells. Total cellular RNA isolated from MNV-1 infected cells was subjected to the RT-PCR amplification as described in method section. Results show that the BV-2, RAW 264.7 and TIB cell lines were infected by MNV-1 (as evident by the appearance RT-PCR product), while CHME-5 appears to be refractory to MNV-1 infection. Lanes: 1 = 100 bp marker, 2 = MNV-infected BV-2 cells, 3 = MNV-infected RAW 264.7 cells, 4 = MNV-infected TIB cells, 5 = infected CHME-5 cells, and 6 = Negative control (ddH2O).