Figure 4
From: Chimeric recombinant rotavirus-like particles as a vehicle for the display of heterologous epitopes
Characterization of recombinant VLPs VP6 171 /V5. A) Sandwich ELISA to detect epitope exposition onto recombinant VLPs surface. Purified wt and recombinant VLPs were captured by polyclonal sera against SA11 strain, then were detected by a monoclonal antibody anti-V5 peptide or a polyclonal mouse sera against VP6 protein. B) Microscopy electronic and immunogold labeled. Purified recombinant VLPs VP6171/V5 were loaded onto nickel grids were incubated with a MAb anti-V5 and then labeled with a rabbit anti-mouse conjugated with gold.