Figure 4

A Expression of pET-UL31 in E. coil and its specificity by Western blot. (a) E. Coil cells harbouring pET 32-UL31 were grown in the absence (1) or presnece (2) of IPTG. (b, c) Specificity of rabbit polyclonal antiserum against the His-UL31 fusion protein. E. Coil cells harbouring pET 32-UL31 were uninduced (3, 5) or induced (4, 6) with IPTG. Proteins were separated by SDS-PAGE and transferred to PVDF membranes. The membranes were incubated with the UL31 antiserum (3, 4) and preimmune rabbit serum (5, 6). Arrowheads indicate the UL31 fusion protein. B Identifiction of the UL31 protein by Western blot. DEF cells were mock-infected (1, 3) or infected with DEV (2, 4) and harvested at 36 h postinfection (p.i.). Proteins were separated by SDS-PAGE and stained with Coomasie brilliant blue (e). UL31 antiserum was used to identify the UL31 protein (f). Molecular mass markers (in kDa) are shown to the left (lane M). Arrowhead indicate the UL31 protein.