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Table 1 List of ewes experimentally infected with pestivirus (BDV-genotype 4) along with their progeny and a summary of qualitative and quantitative results of qRT-PCR in placentae, and blood and tissues from the offspring

From: Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny

Groupa

Lambingb (Ewe ID)

PLACENTAE

PROGENY

  

No.

qRT-PCR

(No. copies)

ID

Condition

Autolysis

Ab ELISAe

qRT-PCR blood (No. copies/ml blood)f

qRT-PCR tissues (Mean No. copies)

No. tissues POS/tissues tested

Tissue with highest RNA copy number

A

T (77781)

1

Neg (0)

1

Foetus

+

Neg

Neg

Pos (63.9)

3/6

Thyroid

(108)

   

2

Foetus

+

Neg

Neg

Pos (52.8)

2/4

Kidney

    

3

Foetus

+

Neg

Neg

Pos (86.1)

3/7

Kidney

 

S (86348)

1

Posc

4

Live lamb

-

Neg

Pos (360597.6)

Pos (36830.4)

10/10

Thyroid

 

D (87632)

2

Posc

5

Live lamb

-

Neg

Pos (1015.6)

Pos (2771.6)

5/10

Kidney

   

Posc

6

Live lamb

-

Pos

Neg (0.0)

Neg (0)

0/10

--

 

D (89395)

1

NT

7

Live lamb

-

Neg

Pos (1421.9)

Neg (0)

0/10

--

    

8

Live lamb

-

Pos

Pos (595.1)

Pos (133.8)

4/10

Skin

 

S (82327)

1

Pos (1075.6)

9

Live lamb

-

CA

Pos (1016.9)

Pos (16.2)

3/11

Mesencephalon

B

T (87650)

1

Pos (5911.5)

10

Stillborn

-

Neg

Pos

Pos (21102.2)

7/7

Skin

(76)

   

11

Stillborn

+

Neg

Pos

Pos (69652.4)

8/8

Kidney

    

12

Live lamb

-

Neg

Pos (1871601.6)

Pos (53731.6)

8/9

cerebellum

 

T (77776)

2

Pos (71826.0)

13

Stillborn

-

Pos

Pos (9932.0)

Pos (23811.9)

10/10

Lymph node

   

Pos (103529.6)

14

Stillborn

-

Pos

Pos (1225.3)

Neg (0)

0/10

--

    

15

Stillborn

-

Pos

Pos (10230.2)

Pos (348.0)

5/10

Skin

 

D (87630)

1

Pos (24294.6)

16

Live lamb

-

Pos

Neg (0.0)

Neg (0)

0/10

--

    

17

Live lamb

-

Pos

Pos (2033.5)

Neg (0)

0/10

--

 

S (86330)

1

Pos (2380.1)

18

Live lamb

-

CA

Pos (1129.8)

Pos (195.0)

1/11

Kidney

 

D (77765)

1

Pos (2496.7)

19

Live lamb

-

Neg

Pos (1964.0)

Neg (0)

0/10

--

    

20

Live lamb

-

Neg

Pos (1279244.8)

Pos (61151.4)

9/10

Cortex

C

S (77770)

1

Pos (53502.4)

21

Live lamb

-

Neg

Pos (73004.4)

Pos (59714.0)

10/10

Cortex

(55)

S (82314)

1

Pos (8196.0)

22

Live lamb

-

CA

Pos (1044864.0)

Pos (225808.4)

9/9

Cortex

 

T (82338)

2

Pos (39392.4)

23

Live lamb

-

Neg

Pos (414482.4)

Pos (65253.0)

10/10

Thymus

    

24

Stillborn

-

Neg

Pos (368889.6)

Pos (103645.6)

10/10

Skin

   

Neg (0)d

25

Stillborn

+

Neg

Pos

Pos (32014.7)

8/8

Skin

  1. NT, Not tested; CA, Colostral antibodies
  2. a In brackets, day of gestation when pestivirus was inoculated
  3. b T, triplet; D, double; S, single
  4. c Three placentas could not be assigned to the corresponding ewe; estimates of viral genome copy numbers were 820.1, 45176.6 and 11604.94
  5. d Partial inhibition
  6. e As previously reported[7]
  7. f Number of copies per ml blood from live lambs or cardiac blood from stillborns; quantitative data not provided for degraded cardiac blood or in the case of foetal fluids