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Figure 3 | Virology Journal

Figure 3

From: Inhibition of Monkeypox virus replication by RNA interference

Figure 3

Dose-response of siA6-a and siE8-d. MK2 cells were transfected with either siA6-a or siE8-d at six different concentrations ranging between 40 to 1.25 nm. Transfected cells were infected with MPV and viral titer was identified at 48 h PI by plaque assay. Results (bars) are expressed as a ratio of the average plaque counts from treated wells to counts in parallel mock-transfected cells ± one standard deviation. Toxicity of both constructs were examined under identical conditions using (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) in MTT assay. Results (lines) are normalized to viability of mock-transfected control cells (secondary Y-axis). Transfected cells maintained over 90% of their viability across tested range of siRNA concentrations, highlighting siRNA specificity and low toxicity.

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